Defect in regulated secretion of P-selectin affects leukocyte recruitment in von Willebrand factor-deficient mice
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چکیده
منابع مشابه
Localized reduction of atherosclerosis in von Willebrand factor-deficient mice.
To examine the role of the platelet adhesion molecule von Willebrand factor (vWf) in atherogenesis, vWf-deficient mice (vWf-/-) were bred with mice lacking the low-density lipoprotein receptor (LDLR-/-) on a C57BL/6J background. LDLR-/-vWf+/+ and LDLR-/-vWf-/- mice were placed on a diet rich in saturated fat and cholesterol for different lengths of time. The atherogenic diet stimulated leukocyt...
متن کاملCorrection of bleeding symptoms in von Willebrand factor-deficient mice by liver-expressed von Willebrand factor mutants.
OBJECTIVE von Willebrand Factor (vWF) structure-function relationship has been studied only in vitro. To investigate the physiological importance of particular vWF domains, we have introduced mutations into murine vWF (mvWF) cDNA inhibiting vWF binding to glycoprotein (Gp) Ib, GpIIbIIIa, and to fibrillar collagen. METHODS AND RESULTS We delivered wild-type (WT) or mutant mvWF cDNA into vWF-de...
متن کاملP-selectin glycoprotein ligand-1-deficient mice have impaired leukocyte tethering to E-selectin under flow.
P-selectin glycoprotein ligand-1 (PSGL-1) mediates rolling of leukocytes on P-selectin under flow. The glycoproteins that enable leukocyte tethering to or rolling on E-selectin are not known. We used gene targeting to prepare PSGL-1-deficient (PSGL-1-/-) mice, which were healthy but had moderately elevated total blood leukocytes. Fluid-phase E-selectin bound to approximately 70% fewer sites on ...
متن کاملDifferential role of von Willebrand factor and P-selectin on microvascular thrombosis in endotoxemia.
OBJECTIVE Endotoxin (lipopolysaccharide [LPS]) enhances microvascular thrombosis in mouse cremaster venules. Because von Willebrand factor (vWF) and P-selectin are suggested to mediate LPS-induced platelet-microvessel interactions, we determined whether vWF and P-selectin contribute to microvascular thrombosis in endotoxemia. METHODS AND RESULTS A light/dye-induced thrombosis model was used i...
متن کاملAnalysis of intracellular storage and regulated secretion of 3 von Willebrand disease-causing variants of von Willebrand factor.
The rapid exocytosis of von Willebrand factor (VWF) in response to vascular injury can be attributed to the fact that VWF is stored in the Weibel-Palade bodies (WPBs) of endothelial cells. We describe a system for examining the ability of VWF to drive both the formation of a storage compartment and the function of that compartment with respect to regulated secretion. Transient transfection of H...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2001
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.061307098